SECTION 1. Section 5 of chapter 334 of the acts of 2016 is hereby amended, in section 4, by inserting the following subparagraph: 

(l) In addition to the license, registration, and monetary penalties collected pursuant to this chapter, the commission shall assess an annual fee of not less than 2,000,000 in proportional shares against each licensed marijuana retail store, product manufacturer, and cultivator in proportion to the gross annual income earned by each establishment. Such fees shall be deposited into the Prevention and Wellness Trust Fund, established in section 2G of Chapter 111, for the purposes of funding evidence-based public health programs dedicated to universal youth substance use prevention. The first assessment of such fee shall occur two years following when the combined total of actively licensed retail stores, manufacturers, cultivators reaches 1,000 establishments.